CONGRATULATIONS TO OUR NEW DOCTOR, Natalya Jukovskaya who graduated with the Very Honorable grade !


Natalya Jukovskaya presented her PhD defense on December 14th at the CRI,Paris Descartes University, Faculty of medicine.

PhD title : "Peripheral adaptation to odors in the rodent olfactory bulb: recordings and consequences on the local functional hyperemia"

Abstract: Functional Magnetic Resonance Imaging technique (fMRI) uses hemodynamic changes in the brain for mapping neuronal activity. Although it is recognized that functional hyperemia is triggered by neurotransmitter release rather than energy consumption by activated neurons, the mechanisms which link neuronal activity and hemodynamic changes are still under debate. We used the olfactory bulb as a biological model to study the relationship between the amount of locally released glutamate from olfactory receptor neuron terminals and blood flow changes in capillaries. We used two-photon laser scanning microscopy to measure in parallel with high spatial and temporal resolution vascular responses in individual capillaries and Ca2+ variations in the terminals of Olfactory Sensory Neurons (OSN).
Here we report that functional hyperemia is positively or negatively coupled to the amount of the locally released glutamate as measured for Ca2+ signals, depending on the absence or the presence of peripheral adaptation. In parallel we investigated the extent for which OSNs are activated homogeneously. We report that during peripheral adaptation OSN terminals are not homogeneously activated.

Keywords: Olfactory bulb, neurovascular coupling, calcium imaging, two-photon microscopy, in vivo, blood flow.